IFI-A · Pectin-Tannin Matrix

Specimen SS-F-AST-522 // ASTRINGENCY · IFI-A

Pectin-Tannin Matrix I-AG-B

Vitis vinifera

Grape

Tier IPectin-Tannin Matrix

Target Trait

1.61×

fold-change

Matrix-bound tannin mouthfeel sustained-release coefficient

Technical Specifications

SKU

SS-F-AST-522

Genomic Edits

4

GC Content

45.8%

Construct Length

2,825 bp

Promoter System

Tissue-specific 2A11

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

Polyphenol-astringency axis

IFI Score

161

Pathway Edits

  • PME overexpression (pectin methylesterase) for cell wall pectin demethylation and tannin association
  • PG1 upregulation (polygalacturonase 1) for pectin architecture remodeling
  • BAHD-GT glucosyltransferase amplification for polysaccharide-tannin linker formation
  • XTH co-expression (xyloglucan endotransglucosylase-hydrolase) for xyloglucan matrix optimization
  • T-DNA right-border optimized integration cassette
  • Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

PME-mediated pectin demethylation for enhanced cell wall tannin associationPG1/BAHD-GT matrix remodeling for sustained mouthfeel tannin presentationXTH xyloglucan restructuring for optimized polysaccharide-tannin co-architectureAgrobacterium co-cultivation with acetosyringone inductionVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
161
IFI-F
—

Use Case

Cell wall-tannin interaction research, entry-level mouthfeel modulation studies, academic pectin-polyphenol interaction analysis. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$349 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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