Polyphenol Oxidase Modulation V-AG-B

Camellia sinensis

Tea

Tier VPolyphenol Oxidase Modulation

Target Trait

3.45×

fold-change

PPO activity-controlled oxidative astringency modulation index

Technical Specifications

SKU

SS-F-AST-514

Genomic Edits

GC Content

47.5%

Construct Length

4,333 bp

Promoter System

Tissue-specific 2A11

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

Polyphenol-astringency axis

IFI Score

345

Pathway Edits

PPO1-alt overexpression (activity-enhanced polyphenol oxidase allele) for controlled catechin oxidation
PPO2-RNAi silencing to suppress uncontrolled browning from PPO2 activity
LAC1 laccase upregulation for complementary catechin ortho-quinone formation
TDC co-expression (tryptophan decarboxylase) for auxiliary oxidative substrate channeling
T-DNA right-border optimized integration cassette
Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

PPO1/PPO2 activity ratio control for precision-managed catechin oxidative couplingLAC1 laccase complementation for expanded oxidative astringency architecturePPO2-RNAi browning suppression for astringency enhancement without oxidative browning artifactsAgrobacterium co-cultivation with acetosyringone inductionVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

345

IFI-F

—

Use Case

Flagship black tea analog ingredient production, ultra-high oxidative astringency standardization, premium tea processing ingredient manufacturing. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$10049 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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