IFI-A · Polyphenol Oxidase Modulation
Specimen SS-F-AST-511 // ASTRINGENCY · IFI-A
Polyphenol Oxidase Modulation IV-IV-A
Camellia sinensis
Tea
Tier IVPolyphenol Oxidase Modulation
Target Trait
2.55×
fold-change
PPO activity-controlled oxidative astringency modulation index
Technical Specifications
SKU
SS-F-AST-511
Genomic Edits
6
GC Content
44.8%
Construct Length
3,491 bp
Promoter System
T7/SP6 cell-free
Expression System
In-vitro cell-free
Host Strain
Cell-free
Receptor Target
Polyphenol-astringency axis
IFI Score
255
Pathway Edits
- PPO1-alt overexpression (activity-enhanced polyphenol oxidase allele) for controlled catechin oxidation
- PPO2-RNAi silencing to suppress uncontrolled browning from PPO2 activity
- LAC1 laccase upregulation for complementary catechin ortho-quinone formation
- TDC co-expression (tryptophan decarboxylase) for auxiliary oxidative substrate channeling
- Cell-free wheat germ extract reconstitution system
Key Interventions
PPO1/PPO2 activity ratio control for precision-managed catechin oxidative couplingLAC1 laccase complementation for expanded oxidative astringency architecturePPO2-RNAi browning suppression for astringency enhancement without oxidative browning artifactsSP6/T7 dual-polymerase cell-free transcription/translation
IFI Subscale Profile
IFI Profile
IFI-S—
IFI-U—
IFI-B—
IFI-K—
IFI-C—
IFI-P—
IFI-A255
IFI-F—
Use Case
Industrial oxidative tea astringency ingredient production, premium black tea analog manufacturing, beverage processing R&D.
License
$3999 · Tier IV License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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