IFI-A · Polyphenol Oxidase Modulation

Specimen SS-F-AST-506 // ASTRINGENCY · IFI-A

Polyphenol Oxidase Modulation IV-AG-B

Camellia sinensis

Tea

Tier IVPolyphenol Oxidase Modulation

Target Trait

2.93×

fold-change

PPO activity-controlled oxidative astringency modulation index

Technical Specifications

SKU

SS-F-AST-506

Genomic Edits

7

GC Content

46.7%

Construct Length

3,986 bp

Promoter System

Tissue-specific 2A11

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

Polyphenol-astringency axis

IFI Score

293

Pathway Edits

  • PPO1-alt overexpression (activity-enhanced polyphenol oxidase allele) for controlled catechin oxidation
  • PPO2-RNAi silencing to suppress uncontrolled browning from PPO2 activity
  • LAC1 laccase upregulation for complementary catechin ortho-quinone formation
  • TDC co-expression (tryptophan decarboxylase) for auxiliary oxidative substrate channeling
  • T-DNA right-border optimized integration cassette
  • Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

PPO1/PPO2 activity ratio control for precision-managed catechin oxidative couplingLAC1 laccase complementation for expanded oxidative astringency architecturePPO2-RNAi browning suppression for astringency enhancement without oxidative browning artifactsAgrobacterium co-cultivation with acetosyringone inductionVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
293
IFI-F
—

Use Case

Industrial oxidative tea astringency ingredient production, premium black tea analog manufacturing, beverage processing R&D. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$4049 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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