IFI-A · Polyphenol Oxidase Modulation

Specimen SS-F-AST-502 // ASTRINGENCY · IFI-A

Polyphenol Oxidase Modulation III-PR-B

Camellia sinensis

Tea

Tier IIIPolyphenol Oxidase Modulation

Target Trait

2.42×

fold-change

PPO activity-controlled oxidative astringency modulation index

Technical Specifications

SKU

SS-F-AST-502

Genomic Edits

6

GC Content

47.6%

Construct Length

3,507 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

Polyphenol-astringency axis

IFI Score

242

Pathway Edits

  • PPO1-alt overexpression (activity-enhanced polyphenol oxidase allele) for controlled catechin oxidation
  • PPO2-RNAi silencing to suppress uncontrolled browning from PPO2 activity
  • LAC1 laccase upregulation for complementary catechin ortho-quinone formation
  • TDC co-expression (tryptophan decarboxylase) for auxiliary oxidative substrate channeling
  • Protoplast PEG-fusion transient and stable integration protocol
  • Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

PPO1/PPO2 activity ratio control for precision-managed catechin oxidative couplingLAC1 laccase complementation for expanded oxidative astringency architecturePPO2-RNAi browning suppression for astringency enhancement without oxidative browning artifactsPEG-4000 mediated membrane fusion at 37°CVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
242
IFI-F
—

Use Case

Commercial PPO-controlled tea ingredient production, mid-range oxidative astringency R&D, beverage black tea analog formulation. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$1549 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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