IFI-A · Polyphenol Oxidase Modulation

Specimen SS-F-AST-488 // ASTRINGENCY · IFI-A

Polyphenol Oxidase Modulation I-IV-B

Camellia sinensis

Tea

Tier IPolyphenol Oxidase Modulation

Target Trait

1.38×

fold-change

PPO activity-controlled oxidative astringency modulation index

Technical Specifications

SKU

SS-F-AST-488

Genomic Edits

4

GC Content

46.0%

Construct Length

2,847 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

Polyphenol-astringency axis

IFI Score

138

Pathway Edits

  • PPO1-alt overexpression (activity-enhanced polyphenol oxidase allele) for controlled catechin oxidation
  • PPO2-RNAi silencing to suppress uncontrolled browning from PPO2 activity
  • LAC1 laccase upregulation for complementary catechin ortho-quinone formation
  • TDC co-expression (tryptophan decarboxylase) for auxiliary oxidative substrate channeling
  • Cell-free wheat germ extract reconstitution system
  • Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

PPO1/PPO2 activity ratio control for precision-managed catechin oxidative couplingLAC1 laccase complementation for expanded oxidative astringency architecturePPO2-RNAi browning suppression for astringency enhancement without oxidative browning artifactsSP6/T7 dual-polymerase cell-free transcription/translationVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
138
IFI-F
—

Use Case

Tea oxidation pathway research, entry-level PPO activity engineering, academic catechin oxidation studies. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$349 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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