IFI-A · Polyphenol Oxidase Modulation

Specimen SS-F-AST-481 // ASTRINGENCY · IFI-A

Polyphenol Oxidase Modulation I-AG-A

Camellia sinensis

Tea

Tier IPolyphenol Oxidase Modulation

Target Trait

1.2×

fold-change

PPO activity-controlled oxidative astringency modulation index

Technical Specifications

SKU

SS-F-AST-481

Genomic Edits

3

GC Content

44.2%

Construct Length

2,348 bp

Promoter System

Tissue-specific 2A11

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

Polyphenol-astringency axis

IFI Score

120

Pathway Edits

  • PPO1-alt overexpression (activity-enhanced polyphenol oxidase allele) for controlled catechin oxidation
  • PPO2-RNAi silencing to suppress uncontrolled browning from PPO2 activity
  • LAC1 laccase upregulation for complementary catechin ortho-quinone formation
  • TDC co-expression (tryptophan decarboxylase) for auxiliary oxidative substrate channeling
  • T-DNA right-border optimized integration cassette

Key Interventions

PPO1/PPO2 activity ratio control for precision-managed catechin oxidative couplingLAC1 laccase complementation for expanded oxidative astringency architecturePPO2-RNAi browning suppression for astringency enhancement without oxidative browning artifactsAgrobacterium co-cultivation with acetosyringone induction

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
120
IFI-F
—

Use Case

Tea oxidation pathway research, entry-level PPO activity engineering, academic catechin oxidation studies.

License

$299 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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