IFI-A · Quercetin-Myricetin

Specimen SS-F-AST-480 // ASTRINGENCY · IFI-A

Quercetin-Myricetin V-IV-B

Vitis vinifera

Grape

Tier VQuercetin-Myricetin

Target Trait

3.79×

fold-change

Quercetin and myricetin flavonol accumulation

Technical Specifications

SKU

SS-F-AST-480

Genomic Edits

8

GC Content

47.4%

Construct Length

4,355 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

Polyphenol-astringency axis

IFI Score

379

Pathway Edits

  • FLS1 overexpression (flavonol synthase 1) for quercetin and myricetin production
  • F3H upregulation (flavanone 3-hydroxylase) for dihydroquercetin precursor supply
  • F3-5H co-expression for myricetin branch from dihydromyricetin
  • CHS amplification (chalcone synthase) for naringenin chalcone substrate expansion
  • CHI enhancement (chalcone isomerase) for naringenin isomerization efficiency
  • Cell-free wheat germ extract reconstitution system
  • Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

FLS1 diversion of dihydroflavonol intermediates toward quercetin/myricetin accumulationCHS/CHI substrate pool expansion for elevated flavonol precursor availabilityF3-5H hydroxylation for myricetin enrichment alongside quercetin productionSP6/T7 dual-polymerase cell-free transcription/translationVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
379
IFI-F
—

Use Case

Flagship flavonol ingredient production, pharmaceutical-grade quercetin standardization, premium nutraceutical grape extract manufacturing. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$10049 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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