Prodelphindin Enhancement II-IV-B

Vaccinium corymbosum

Blueberry

Tier IIProdelphindin Enhancement

Target Trait

2.24×

fold-change

Gallocatechin-based proanthocyanidin (prodelphindin) accumulation

Technical Specifications

SKU

SS-F-AST-416

Genomic Edits

GC Content

47.3%

Construct Length

3,234 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

Polyphenol-astringency axis

IFI Score

224

Pathway Edits

F3-5H overexpression (flavonoid 3',5'-hydroxylase) for 3,5-dihydroxyl ring B substitution
F3H upregulation (flavanone 3-hydroxylase) for dihydromyricetin precursor supply
ANR co-activation (anthocyanidin reductase) for gallocatechin extension unit formation
LAR alternative allele for leucodelphinidin reductase activity toward gallocatechin
DFR-alt selection for leucodelphinidin substrate preference over leucocyanidin
Cell-free wheat germ extract reconstitution system
Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

F3-5H hydroxylation shift to 3',5'-dihydroxyl for prodelphindin unit biosynthesisGallocatechin precursor pool expansion via F3H/ANR co-activationDFR-alt allelic selection for prodelphindin branch preference over procyanidin pathwaySP6/T7 dual-polymerase cell-free transcription/translationVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

224

IFI-F

—

Use Case

Craft high-prodelphindin blueberry extract development, specialty functional ingredient sourcing. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$749 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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