IFI-A · Prodelphindin Enhancement
Specimen SS-F-AST-415 // ASTRINGENCY · IFI-A
Prodelphindin Enhancement II-IV-A
Vaccinium corymbosum
Blueberry
Tier IIProdelphindin Enhancement
Target Trait
1.95×
fold-change
Gallocatechin-based proanthocyanidin (prodelphindin) accumulation
Technical Specifications
SKU
SS-F-AST-415
Genomic Edits
4
GC Content
45.4%
Construct Length
2,837 bp
Promoter System
T7/SP6 cell-free
Expression System
In-vitro cell-free
Host Strain
Cell-free
Receptor Target
Polyphenol-astringency axis
IFI Score
195
Pathway Edits
- F3-5H overexpression (flavonoid 3',5'-hydroxylase) for 3,5-dihydroxyl ring B substitution
- F3H upregulation (flavanone 3-hydroxylase) for dihydromyricetin precursor supply
- ANR co-activation (anthocyanidin reductase) for gallocatechin extension unit formation
- LAR alternative allele for leucodelphinidin reductase activity toward gallocatechin
- DFR-alt selection for leucodelphinidin substrate preference over leucocyanidin
- Cell-free wheat germ extract reconstitution system
Key Interventions
F3-5H hydroxylation shift to 3',5'-dihydroxyl for prodelphindin unit biosynthesisGallocatechin precursor pool expansion via F3H/ANR co-activationDFR-alt allelic selection for prodelphindin branch preference over procyanidin pathwaySP6/T7 dual-polymerase cell-free transcription/translation
IFI Subscale Profile
IFI Profile
IFI-S—
IFI-U—
IFI-B—
IFI-K—
IFI-C—
IFI-P—
IFI-A195
IFI-F—
Use Case
Craft high-prodelphindin blueberry extract development, specialty functional ingredient sourcing.
License
$699 · Tier II License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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