Specimen SS-F-AST-408 // ASTRINGENCY · IFI-A
Prodelphindin Enhancement I-IV-B
Vaccinium corymbosum
Blueberry
Target Trait
1.61×
fold-change
Gallocatechin-based proanthocyanidin (prodelphindin) accumulation
Technical Specifications
SKU
SS-F-AST-408
Genomic Edits
4
GC Content
46.0%
Construct Length
2,887 bp
Promoter System
T7/SP6 cell-free
Expression System
In-vitro cell-free
Host Strain
Cell-free
Receptor Target
Polyphenol-astringency axis
IFI Score
161
Pathway Edits
- F3-5H overexpression (flavonoid 3',5'-hydroxylase) for 3,5-dihydroxyl ring B substitution
- F3H upregulation (flavanone 3-hydroxylase) for dihydromyricetin precursor supply
- ANR co-activation (anthocyanidin reductase) for gallocatechin extension unit formation
- LAR alternative allele for leucodelphinidin reductase activity toward gallocatechin
- DFR-alt selection for leucodelphinidin substrate preference over leucocyanidin
- Cell-free wheat germ extract reconstitution system
- Enhanced flux via 5'UTR synthetic leader sequence optimization
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Prodelphindin pathway research, blueberry polyphenol profile studies, entry-level PA hydroxylation engineering. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.
License
$349 · Tier I License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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