IFI-A · Prodelphindin Enhancement

Specimen SS-F-AST-405 // ASTRINGENCY · IFI-A

Prodelphindin Enhancement I-PR-A

Vaccinium corymbosum

Blueberry

Tier IProdelphindin Enhancement

Target Trait

1.4×

fold-change

Gallocatechin-based proanthocyanidin (prodelphindin) accumulation

Technical Specifications

SKU

SS-F-AST-405

Genomic Edits

3

GC Content

43.9%

Construct Length

2,456 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

Polyphenol-astringency axis

IFI Score

140

Pathway Edits

  • F3-5H overexpression (flavonoid 3',5'-hydroxylase) for 3,5-dihydroxyl ring B substitution
  • F3H upregulation (flavanone 3-hydroxylase) for dihydromyricetin precursor supply
  • ANR co-activation (anthocyanidin reductase) for gallocatechin extension unit formation
  • LAR alternative allele for leucodelphinidin reductase activity toward gallocatechin
  • DFR-alt selection for leucodelphinidin substrate preference over leucocyanidin
  • Protoplast PEG-fusion transient and stable integration protocol

Key Interventions

F3-5H hydroxylation shift to 3',5'-dihydroxyl for prodelphindin unit biosynthesisGallocatechin precursor pool expansion via F3H/ANR co-activationDFR-alt allelic selection for prodelphindin branch preference over procyanidin pathwayPEG-4000 mediated membrane fusion at 37°C

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
140
IFI-F
—

Use Case

Prodelphindin pathway research, blueberry polyphenol profile studies, entry-level PA hydroxylation engineering.

License

$299 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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