Specimen SS-F-AST-400 // ASTRINGENCY · IFI-A
Anthocyanin-Tannin Co-Engineering V-IV-B
Vitis vinifera
Grape
Target Trait
4.6×
fold-change
Anthocyanin-tannin co-pigmentation architecture index
Technical Specifications
SKU
SS-F-AST-400
Genomic Edits
10
GC Content
47.4%
Construct Length
5,155 bp
Promoter System
T7/SP6 cell-free
Expression System
In-vitro cell-free
Host Strain
Cell-free
Receptor Target
Polyphenol-astringency axis
IFI Score
460
Pathway Edits
- ANS overexpression (anthocyanidin synthase) for parallel anthocyanin formation
- UFGT amplification (UDP-glucose flavonoid 3-O-glucosyltransferase) for anthocyanin glycosylation
- LAR co-activation (leucoanthocyanidin reductase) for proanthocyanidin branch supply
- ANR upregulation (anthocyanidin reductase) for epicatechin PA extension units
- TT12 vacuolar transport for co-localization of tannin and anthocyanin in vacuole
- Cell-free wheat germ extract reconstitution system
- Enhanced flux via 5'UTR synthetic leader sequence optimization
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Flagship premium wine-style beverage engineering, ultra-premium tannin-anthocyanin co-ingredient production, next-generation functional beverage flavor architecture. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.
License
$10049 · Tier V License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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