IFI-A · Anthocyanin-Tannin Co-Engineering

Specimen SS-F-AST-391 // ASTRINGENCY · IFI-A

Anthocyanin-Tannin Co-Engineering IV-IV-A

Vitis vinifera

Grape

Tier IVAnthocyanin-Tannin Co-Engineering

Target Trait

3.4×

fold-change

Anthocyanin-tannin co-pigmentation architecture index

Technical Specifications

SKU

SS-F-AST-391

Genomic Edits

8

GC Content

44.8%

Construct Length

4,411 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

Polyphenol-astringency axis

IFI Score

340

Pathway Edits

  • ANS overexpression (anthocyanidin synthase) for parallel anthocyanin formation
  • UFGT amplification (UDP-glucose flavonoid 3-O-glucosyltransferase) for anthocyanin glycosylation
  • LAR co-activation (leucoanthocyanidin reductase) for proanthocyanidin branch supply
  • ANR upregulation (anthocyanidin reductase) for epicatechin PA extension units
  • TT12 vacuolar transport for co-localization of tannin and anthocyanin in vacuole
  • Cell-free wheat germ extract reconstitution system

Key Interventions

Anthocyanin and proanthocyanidin branch co-activation for co-pigmentation architectureTT12 vacuolar sequestration for tannin-anthocyanin co-compartmentalizationColor-astringency integration via ANS/UFGT/LAR/ANR balanced flux engineeringSP6/T7 dual-polymerase cell-free transcription/translation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
340
IFI-F
—

Use Case

Industrial wine-style co-pigmentation ingredient production, premium beverage flavor architecture engineering.

License

$3999 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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