Anthocyanin-Tannin Co-Engineering IV-BL-B

Vitis vinifera

Grape

Tier IVAnthocyanin-Tannin Co-Engineering

Target Trait

3.91×

fold-change

Anthocyanin-tannin co-pigmentation architecture index

Technical Specifications

SKU

SS-F-AST-388

Genomic Edits

GC Content

47.0%

Construct Length

4,740 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic transformation

Host Strain

Particle Gun

Receptor Target

Polyphenol-astringency axis

IFI Score

391

Pathway Edits

ANS overexpression (anthocyanidin synthase) for parallel anthocyanin formation
UFGT amplification (UDP-glucose flavonoid 3-O-glucosyltransferase) for anthocyanin glycosylation
LAR co-activation (leucoanthocyanidin reductase) for proanthocyanidin branch supply
ANR upregulation (anthocyanidin reductase) for epicatechin PA extension units
TT12 vacuolar transport for co-localization of tannin and anthocyanin in vacuole
Biolistic tungsten-particle coating for leaf/seed explant delivery
Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

Anthocyanin and proanthocyanidin branch co-activation for co-pigmentation architectureTT12 vacuolar sequestration for tannin-anthocyanin co-compartmentalizationColor-astringency integration via ANS/UFGT/LAR/ANR balanced flux engineeringHigh-pressure helium particle acceleration at 1100 psiVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

391

IFI-F

—

Use Case

Industrial wine-style co-pigmentation ingredient production, premium beverage flavor architecture engineering. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$4049 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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