Anthocyanin-Tannin Co-Engineering III-IV-B

Vitis vinifera

Grape

Tier IIIAnthocyanin-Tannin Co-Engineering

Target Trait

3.22×

fold-change

Anthocyanin-tannin co-pigmentation architecture index

Technical Specifications

SKU

SS-F-AST-384

Genomic Edits

GC Content

48.9%

Construct Length

4,261 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

Polyphenol-astringency axis

IFI Score

322

Pathway Edits

ANS overexpression (anthocyanidin synthase) for parallel anthocyanin formation
UFGT amplification (UDP-glucose flavonoid 3-O-glucosyltransferase) for anthocyanin glycosylation
LAR co-activation (leucoanthocyanidin reductase) for proanthocyanidin branch supply
ANR upregulation (anthocyanidin reductase) for epicatechin PA extension units
TT12 vacuolar transport for co-localization of tannin and anthocyanin in vacuole
Cell-free wheat germ extract reconstitution system
Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

Anthocyanin and proanthocyanidin branch co-activation for co-pigmentation architectureTT12 vacuolar sequestration for tannin-anthocyanin co-compartmentalizationColor-astringency integration via ANS/UFGT/LAR/ANR balanced flux engineeringSP6/T7 dual-polymerase cell-free transcription/translationVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

322

IFI-F

—

Use Case

Commercial co-pigmentation ingredient production, mid-range wine analog manufacturing, functional beverage color-astringency integration. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$1549 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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