IFI-A · Anthocyanin-Tannin Co-Engineering

Specimen SS-F-AST-383 // ASTRINGENCY · IFI-A

Anthocyanin-Tannin Co-Engineering III-IV-A

Vitis vinifera

Grape

Tier IIIAnthocyanin-Tannin Co-Engineering

Target Trait

2.8×

fold-change

Anthocyanin-tannin co-pigmentation architecture index

Technical Specifications

SKU

SS-F-AST-383

Genomic Edits

7

GC Content

43.8%

Construct Length

3,864 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

Polyphenol-astringency axis

IFI Score

280

Pathway Edits

  • ANS overexpression (anthocyanidin synthase) for parallel anthocyanin formation
  • UFGT amplification (UDP-glucose flavonoid 3-O-glucosyltransferase) for anthocyanin glycosylation
  • LAR co-activation (leucoanthocyanidin reductase) for proanthocyanidin branch supply
  • ANR upregulation (anthocyanidin reductase) for epicatechin PA extension units
  • TT12 vacuolar transport for co-localization of tannin and anthocyanin in vacuole
  • Cell-free wheat germ extract reconstitution system

Key Interventions

Anthocyanin and proanthocyanidin branch co-activation for co-pigmentation architectureTT12 vacuolar sequestration for tannin-anthocyanin co-compartmentalizationColor-astringency integration via ANS/UFGT/LAR/ANR balanced flux engineeringSP6/T7 dual-polymerase cell-free transcription/translation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
280
IFI-F
—

Use Case

Commercial co-pigmentation ingredient production, mid-range wine analog manufacturing, functional beverage color-astringency integration.

License

$1499 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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