IFI-A · Catechin Profile Optimization

Specimen SS-F-AST-337 // ASTRINGENCY · IFI-A

Catechin Profile Optimization III-AG-A

Camellia sinensis

Tea

Tier IIICatechin Profile Optimization

Target Trait

2.45×

fold-change

EGCG:ECG:EC catechin molar ratio index

Technical Specifications

SKU

SS-F-AST-337

Genomic Edits

6

GC Content

43.7%

Construct Length

3,502 bp

Promoter System

Tissue-specific 2A11

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

Polyphenol-astringency axis

IFI Score

245

Pathway Edits

  • ANR differential expression (anthocyanidin reductase) for epicatechin flux control
  • LAR allelic balancing (leucoanthocyanidin reductase) for catechin:epicatechin ratio
  • F3-5H overexpression (flavonoid 3',5'-hydroxylase) for gallated catechin precursor enrichment
  • DFR allelic selection for 3',4'- vs 3',4',5'-dihydroxyl substrate preference
  • FLS suppression (flavonol synthase) to redirect dihydroflavonol flux toward catechins
  • T-DNA right-border optimized integration cassette

Key Interventions

EGCG:ECG:EC catechin ratio precision control via ANR/LAR differential tuningF3-5H hydroxylation pattern optimization for gallated versus non-gallated catechin distributionDFR allelic switching for substrate specificity toward epicatechin pathwayAgrobacterium co-cultivation with acetosyringone induction

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
245
IFI-F
—

Use Case

Commercial catechin ingredient production, mid-range green tea analog manufacturing, nutraceutical catechin R&D.

License

$1499 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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