IFI-A · Catechin Profile Optimization
Specimen SS-F-AST-327 // ASTRINGENCY · IFI-A
Catechin Profile Optimization I-IV-A
Camellia sinensis
Tea
Tier ICatechin Profile Optimization
Target Trait
1.4×
fold-change
EGCG:ECG:EC catechin molar ratio index
Technical Specifications
SKU
SS-F-AST-327
Genomic Edits
3
GC Content
44.7%
Construct Length
2,530 bp
Promoter System
T7/SP6 cell-free
Expression System
In-vitro cell-free
Host Strain
Cell-free
Receptor Target
Polyphenol-astringency axis
IFI Score
140
Pathway Edits
- ANR differential expression (anthocyanidin reductase) for epicatechin flux control
- LAR allelic balancing (leucoanthocyanidin reductase) for catechin:epicatechin ratio
- F3-5H overexpression (flavonoid 3',5'-hydroxylase) for gallated catechin precursor enrichment
- DFR allelic selection for 3',4'- vs 3',4',5'-dihydroxyl substrate preference
- FLS suppression (flavonol synthase) to redirect dihydroflavonol flux toward catechins
- Cell-free wheat germ extract reconstitution system
Key Interventions
EGCG:ECG:EC catechin ratio precision control via ANR/LAR differential tuningF3-5H hydroxylation pattern optimization for gallated versus non-gallated catechin distributionDFR allelic switching for substrate specificity toward epicatechin pathwaySP6/T7 dual-polymerase cell-free transcription/translation
IFI Subscale Profile
IFI Profile
IFI-S—
IFI-U—
IFI-B—
IFI-K—
IFI-C—
IFI-P—
IFI-A140
IFI-F—
Use Case
Green tea catechin ratio research, entry-level EGCG optimization studies, academic tea phytochemistry.
License
$299 · Tier I License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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