Catechin Profile Optimization I-PR-B

Camellia sinensis

Tea

Tier ICatechin Profile Optimization

Target Trait

1.61×

fold-change

EGCG:ECG:EC catechin molar ratio index

Technical Specifications

SKU

SS-F-AST-326

Genomic Edits

GC Content

48.5%

Construct Length

2,893 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

Polyphenol-astringency axis

IFI Score

161

Pathway Edits

ANR differential expression (anthocyanidin reductase) for epicatechin flux control
LAR allelic balancing (leucoanthocyanidin reductase) for catechin:epicatechin ratio
F3-5H overexpression (flavonoid 3',5'-hydroxylase) for gallated catechin precursor enrichment
DFR allelic selection for 3',4'- vs 3',4',5'-dihydroxyl substrate preference
FLS suppression (flavonol synthase) to redirect dihydroflavonol flux toward catechins
Protoplast PEG-fusion transient and stable integration protocol
Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

EGCG:ECG:EC catechin ratio precision control via ANR/LAR differential tuningF3-5H hydroxylation pattern optimization for gallated versus non-gallated catechin distributionDFR allelic switching for substrate specificity toward epicatechin pathwayPEG-4000 mediated membrane fusion at 37°CVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

161

IFI-F

—

Use Case

Green tea catechin ratio research, entry-level EGCG optimization studies, academic tea phytochemistry. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$349 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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