IFI-A · Catechin Profile Optimization

Specimen SS-F-AST-325 // ASTRINGENCY · IFI-A

Catechin Profile Optimization I-PR-A

Camellia sinensis

Tea

Tier ICatechin Profile Optimization

Target Trait

1.4×

fold-change

EGCG:ECG:EC catechin molar ratio index

Technical Specifications

SKU

SS-F-AST-325

Genomic Edits

3

GC Content

43.9%

Construct Length

2,496 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

Polyphenol-astringency axis

IFI Score

140

Pathway Edits

  • ANR differential expression (anthocyanidin reductase) for epicatechin flux control
  • LAR allelic balancing (leucoanthocyanidin reductase) for catechin:epicatechin ratio
  • F3-5H overexpression (flavonoid 3',5'-hydroxylase) for gallated catechin precursor enrichment
  • DFR allelic selection for 3',4'- vs 3',4',5'-dihydroxyl substrate preference
  • FLS suppression (flavonol synthase) to redirect dihydroflavonol flux toward catechins
  • Protoplast PEG-fusion transient and stable integration protocol

Key Interventions

EGCG:ECG:EC catechin ratio precision control via ANR/LAR differential tuningF3-5H hydroxylation pattern optimization for gallated versus non-gallated catechin distributionDFR allelic switching for substrate specificity toward epicatechin pathwayPEG-4000 mediated membrane fusion at 37°C

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
140
IFI-F
—

Use Case

Green tea catechin ratio research, entry-level EGCG optimization studies, academic tea phytochemistry.

License

$299 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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