Ellagitannin Engineering V-BL-B

Punica granatum

Pomegranate

Tier VEllagitannin Engineering

Target Trait

4.83×

fold-change

Ellagic acid/ellagitannin scaffold accumulation

Technical Specifications

SKU

SS-F-AST-316

Genomic Edits

GC Content

48.7%

Construct Length

5,127 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic transformation

Host Strain

Particle Gun

Receptor Target

Polyphenol-astringency axis

IFI Score

483

Pathway Edits

UGGT overexpression (galloyl-UDP-glucosyltransferase) for galloyl-glucose scaffold initiation
laccase-ELG upregulation for oxidative C-C galloyl coupling toward ellagic acid
PGT polygalloylglucoside transferase amplification for ellagitannin polymerization
cytochrome-b5 electron carrier co-expression for laccase-ELG redox support
UGGT/laccase-ELG bicistron optimization for coupled galloylation-oxidation
Biolistic tungsten-particle coating for leaf/seed explant delivery
Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

Galloyl-glucose oxidative coupling cascade via UGGT/laccase-ELGEllagitannin polymerization degree control via PGT activity tuningCytochrome-b5 redox cofactor enhancement for oxidase cascade efficiencyHigh-pressure helium particle acceleration at 1100 psiVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

483

IFI-F

—

Use Case

Flagship pharmaceutical ellagitannin production, premium anti-inflammatory ingredient manufacturing, precision ellagic acid standardization. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$10049 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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