IFI-A · Ellagitannin Engineering

Specimen SS-F-AST-312 // ASTRINGENCY · IFI-A

Ellagitannin Engineering IV-IV-B

Punica granatum

Pomegranate

Tier IVEllagitannin Engineering

Target Trait

4.06×

fold-change

Ellagic acid/ellagitannin scaffold accumulation

Technical Specifications

SKU

SS-F-AST-312

Genomic Edits

9

GC Content

46.2%

Construct Length

4,648 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

Polyphenol-astringency axis

IFI Score

406

Pathway Edits

  • UGGT overexpression (galloyl-UDP-glucosyltransferase) for galloyl-glucose scaffold initiation
  • laccase-ELG upregulation for oxidative C-C galloyl coupling toward ellagic acid
  • PGT polygalloylglucoside transferase amplification for ellagitannin polymerization
  • cytochrome-b5 electron carrier co-expression for laccase-ELG redox support
  • UGGT/laccase-ELG bicistron optimization for coupled galloylation-oxidation
  • Cell-free wheat germ extract reconstitution system
  • Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

Galloyl-glucose oxidative coupling cascade via UGGT/laccase-ELGEllagitannin polymerization degree control via PGT activity tuningCytochrome-b5 redox cofactor enhancement for oxidase cascade efficiencySP6/T7 dual-polymerase cell-free transcription/translationVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
406
IFI-F
—

Use Case

Industrial ellagitannin extraction, premium anti-inflammatory ingredient production, beverage polyphenol scale-up. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$4049 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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