IFI-A · Ellagitannin Engineering
Specimen SS-F-AST-295 // ASTRINGENCY · IFI-A
Ellagitannin Engineering II-IV-A
Punica granatum
Pomegranate
Tier IIEllagitannin Engineering
Target Trait
2.17×
fold-change
Ellagic acid/ellagitannin scaffold accumulation
Technical Specifications
SKU
SS-F-AST-295
Genomic Edits
5
GC Content
45.4%
Construct Length
3,177 bp
Promoter System
T7/SP6 cell-free
Expression System
In-vitro cell-free
Host Strain
Cell-free
Receptor Target
Polyphenol-astringency axis
IFI Score
217
Pathway Edits
- UGGT overexpression (galloyl-UDP-glucosyltransferase) for galloyl-glucose scaffold initiation
- laccase-ELG upregulation for oxidative C-C galloyl coupling toward ellagic acid
- PGT polygalloylglucoside transferase amplification for ellagitannin polymerization
- cytochrome-b5 electron carrier co-expression for laccase-ELG redox support
- UGGT/laccase-ELG bicistron optimization for coupled galloylation-oxidation
- Cell-free wheat germ extract reconstitution system
Key Interventions
Galloyl-glucose oxidative coupling cascade via UGGT/laccase-ELGEllagitannin polymerization degree control via PGT activity tuningCytochrome-b5 redox cofactor enhancement for oxidase cascade efficiencySP6/T7 dual-polymerase cell-free transcription/translation
IFI Subscale Profile
IFI Profile
IFI-S—
IFI-U—
IFI-B—
IFI-K—
IFI-C—
IFI-P—
IFI-A217
IFI-F—
Use Case
Specialty pomegranate extract development, intermediate ellagitannin research, nutraceutical prototype formulation.
License
$699 · Tier II License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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