IFI-A · Ellagitannin Engineering

Specimen SS-F-AST-291 // ASTRINGENCY · IFI-A

Ellagitannin Engineering II-BL-A

Punica granatum

Pomegranate

Tier IIEllagitannin Engineering

Target Trait

2.17×

fold-change

Ellagic acid/ellagitannin scaffold accumulation

Technical Specifications

SKU

SS-F-AST-291

Genomic Edits

5

GC Content

43.5%

Construct Length

3,109 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic transformation

Host Strain

Particle Gun

Receptor Target

Polyphenol-astringency axis

IFI Score

217

Pathway Edits

  • UGGT overexpression (galloyl-UDP-glucosyltransferase) for galloyl-glucose scaffold initiation
  • laccase-ELG upregulation for oxidative C-C galloyl coupling toward ellagic acid
  • PGT polygalloylglucoside transferase amplification for ellagitannin polymerization
  • cytochrome-b5 electron carrier co-expression for laccase-ELG redox support
  • UGGT/laccase-ELG bicistron optimization for coupled galloylation-oxidation
  • Biolistic tungsten-particle coating for leaf/seed explant delivery

Key Interventions

Galloyl-glucose oxidative coupling cascade via UGGT/laccase-ELGEllagitannin polymerization degree control via PGT activity tuningCytochrome-b5 redox cofactor enhancement for oxidase cascade efficiencyHigh-pressure helium particle acceleration at 1100 psi

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
217
IFI-F
—

Use Case

Specialty pomegranate extract development, intermediate ellagitannin research, nutraceutical prototype formulation.

License

$699 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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