IFI-A · Gallic Acid Enhancement

Specimen SS-F-AST-272 // ASTRINGENCY · IFI-A

Gallic Acid Enhancement IV-IV-B

Punica granatum

Pomegranate

Tier IVGallic Acid Enhancement

Target Trait

3.65×

fold-change

Gallic acid (hydrolysable tannin precursor) accumulation

Technical Specifications

SKU

SS-F-AST-272

Genomic Edits

8

GC Content

46.2%

Construct Length

4,388 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

Polyphenol-astringency axis

IFI Score

365

Pathway Edits

  • GAE upregulation (gallate-ester hydrolase) for galloyl group liberation
  • GT1 galloylquinic acid glucosyltransferase amplification
  • BAHD-GalT acyltransferase overexpression for galloyl-glucose ester formation
  • 4CL (4-coumarate:CoA ligase) shikimate branch redirection
  • C4H (cinnamate 4-hydroxylase) phenylpropanoid flux enhancement
  • Cell-free wheat germ extract reconstitution system
  • Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

Shikimate pathway carbon flux redirection toward gallic acid branchGalloyl-glucose ester accumulation for hydrolysable tannin precursor poolPolyphenol galloylation capacity expansion in Punica granatumSP6/T7 dual-polymerase cell-free transcription/translationVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
365
IFI-F
—

Use Case

Industrial ellagic acid extraction, premium pomegranate nutraceutical production, beverage polyphenol ingredient scale-up. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$4049 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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