IFI-A · Gallic Acid Enhancement

Specimen SS-F-AST-254 // ASTRINGENCY · IFI-A

Gallic Acid Enhancement II-PR-B

Punica granatum

Pomegranate

Tier IIGallic Acid Enhancement

Target Trait

2.22×

fold-change

Gallic acid (hydrolysable tannin precursor) accumulation

Technical Specifications

SKU

SS-F-AST-254

Genomic Edits

5

GC Content

46.8%

Construct Length

3,280 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

Polyphenol-astringency axis

IFI Score

222

Pathway Edits

  • GAE upregulation (gallate-ester hydrolase) for galloyl group liberation
  • GT1 galloylquinic acid glucosyltransferase amplification
  • BAHD-GalT acyltransferase overexpression for galloyl-glucose ester formation
  • 4CL (4-coumarate:CoA ligase) shikimate branch redirection
  • C4H (cinnamate 4-hydroxylase) phenylpropanoid flux enhancement
  • Protoplast PEG-fusion transient and stable integration protocol
  • Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

Shikimate pathway carbon flux redirection toward gallic acid branchGalloyl-glucose ester accumulation for hydrolysable tannin precursor poolPolyphenol galloylation capacity expansion in Punica granatumPEG-4000 mediated membrane fusion at 37°CVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
222
IFI-F
—

Use Case

Craft pomegranate extract production, specialty health ingredient sourcing, intermediate ellagitannin research. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$749 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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