IFI-A · Gallic Acid Enhancement
Specimen SS-F-AST-245 // ASTRINGENCY · IFI-A
Gallic Acid Enhancement I-PR-A
Punica granatum
Pomegranate
Tier IGallic Acid Enhancement
Target Trait
1.3×
fold-change
Gallic acid (hydrolysable tannin precursor) accumulation
Technical Specifications
SKU
SS-F-AST-245
Genomic Edits
3
GC Content
43.9%
Construct Length
2,536 bp
Promoter System
Ubiquitin / T7
Expression System
Protoplast PEG-fusion
Host Strain
PEG-mediated
Receptor Target
Polyphenol-astringency axis
IFI Score
130
Pathway Edits
- GAE upregulation (gallate-ester hydrolase) for galloyl group liberation
- GT1 galloylquinic acid glucosyltransferase amplification
- BAHD-GalT acyltransferase overexpression for galloyl-glucose ester formation
- 4CL (4-coumarate:CoA ligase) shikimate branch redirection
- C4H (cinnamate 4-hydroxylase) phenylpropanoid flux enhancement
- Protoplast PEG-fusion transient and stable integration protocol
Key Interventions
Shikimate pathway carbon flux redirection toward gallic acid branchGalloyl-glucose ester accumulation for hydrolysable tannin precursor poolPolyphenol galloylation capacity expansion in Punica granatumPEG-4000 mediated membrane fusion at 37°C
IFI Subscale Profile
IFI Profile
IFI-S—
IFI-U—
IFI-B—
IFI-K—
IFI-C—
IFI-P—
IFI-A130
IFI-F—
Use Case
Research-grade ellagitannin precursor production, pomegranate polyphenol pathway studies, entry-level galloylation screening.
License
$299 · Tier I License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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