Condensed Tannin Degree V-IV-B

Vitis vinifera

Grape

Tier VCondensed Tannin Degree

Target Trait

5.17×

fold-change

Proanthocyanidin degree of polymerization

Technical Specifications

SKU

SS-F-AST-240

Genomic Edits

GC Content

47.4%

Construct Length

5,035 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

Polyphenol-astringency axis

IFI Score

517

Pathway Edits

LAR upregulation (leucoanthocyanidin reductase) for catechin starter unit supply
ANR co-activation (anthocyanidin reductase) for epicatechin extension units
TT12 vacuolar flavonoid transporter amplification
TT19 glutathione S-transferase overexpression for PA vacuolar sequestration
AHA10 H+-ATPase enhancement for vacuolar acidification and tannin stability
Cell-free wheat germ extract reconstitution system
Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

Condensed tannin polymerization degree control via LAR:ANR ratio tuningVacuolar tannin transport and sequestration efficiency optimizationPA mouthfeel modulation through proanthocyanidin chain length engineeringSP6/T7 dual-polymerase cell-free transcription/translationVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

517

IFI-F

—

Use Case

Flagship precision tannin standardization, next-generation wine style engineering, pharmaceutical-grade PA extract production. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$10049 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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