IFI-A · Condensed Tannin Degree

Specimen SS-F-AST-216 // ASTRINGENCY · IFI-A

Condensed Tannin Degree II-IV-B

Vitis vinifera

Grape

Tier IICondensed Tannin Degree

Target Trait

2.59×

fold-change

Proanthocyanidin degree of polymerization

Technical Specifications

SKU

SS-F-AST-216

Genomic Edits

6

GC Content

47.3%

Construct Length

3,614 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

Polyphenol-astringency axis

IFI Score

259

Pathway Edits

  • LAR upregulation (leucoanthocyanidin reductase) for catechin starter unit supply
  • ANR co-activation (anthocyanidin reductase) for epicatechin extension units
  • TT12 vacuolar flavonoid transporter amplification
  • TT19 glutathione S-transferase overexpression for PA vacuolar sequestration
  • AHA10 H+-ATPase enhancement for vacuolar acidification and tannin stability
  • Cell-free wheat germ extract reconstitution system
  • Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

Condensed tannin polymerization degree control via LAR:ANR ratio tuningVacuolar tannin transport and sequestration efficiency optimizationPA mouthfeel modulation through proanthocyanidin chain length engineeringSP6/T7 dual-polymerase cell-free transcription/translationVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
259
IFI-F
—

Use Case

Craft wine and cider tannin grading, specialty food astringency research, beverage R&D ingredient sourcing. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$749 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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