IFI-A · Condensed Tannin Degree

Specimen SS-F-AST-207 // ASTRINGENCY · IFI-A

Condensed Tannin Degree I-IV-A

Vitis vinifera

Grape

Tier ICondensed Tannin Degree

Target Trait

1.5×

fold-change

Proanthocyanidin degree of polymerization

Technical Specifications

SKU

SS-F-AST-207

Genomic Edits

4

GC Content

44.7%

Construct Length

2,870 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

Polyphenol-astringency axis

IFI Score

150

Pathway Edits

  • LAR upregulation (leucoanthocyanidin reductase) for catechin starter unit supply
  • ANR co-activation (anthocyanidin reductase) for epicatechin extension units
  • TT12 vacuolar flavonoid transporter amplification
  • TT19 glutathione S-transferase overexpression for PA vacuolar sequestration
  • AHA10 H+-ATPase enhancement for vacuolar acidification and tannin stability
  • Cell-free wheat germ extract reconstitution system

Key Interventions

Condensed tannin polymerization degree control via LAR:ANR ratio tuningVacuolar tannin transport and sequestration efficiency optimizationPA mouthfeel modulation through proanthocyanidin chain length engineeringSP6/T7 dual-polymerase cell-free transcription/translation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
150
IFI-F
—

Use Case

Proof-of-concept tannin grading, academic soft tannin research, entry-level wine analog mouthfeel development.

License

$299 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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