IFI-A · Condensed Tannin Degree

Specimen SS-F-AST-206 // ASTRINGENCY · IFI-A

Condensed Tannin Degree I-PR-B

Vitis vinifera

Grape

Tier ICondensed Tannin Degree

Target Trait

1.72×

fold-change

Proanthocyanidin degree of polymerization

Technical Specifications

SKU

SS-F-AST-206

Genomic Edits

5

GC Content

48.5%

Construct Length

3,233 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

Polyphenol-astringency axis

IFI Score

172

Pathway Edits

  • LAR upregulation (leucoanthocyanidin reductase) for catechin starter unit supply
  • ANR co-activation (anthocyanidin reductase) for epicatechin extension units
  • TT12 vacuolar flavonoid transporter amplification
  • TT19 glutathione S-transferase overexpression for PA vacuolar sequestration
  • AHA10 H+-ATPase enhancement for vacuolar acidification and tannin stability
  • Protoplast PEG-fusion transient and stable integration protocol
  • Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

Condensed tannin polymerization degree control via LAR:ANR ratio tuningVacuolar tannin transport and sequestration efficiency optimizationPA mouthfeel modulation through proanthocyanidin chain length engineeringPEG-4000 mediated membrane fusion at 37°CVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
172
IFI-F
—

Use Case

Proof-of-concept tannin grading, academic soft tannin research, entry-level wine analog mouthfeel development. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$349 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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