Commercial Access Only
Specimen SS-COM-BEY-1799 // BEYOND-PEPPERS
Pear T5-LF-FD
Pyrus communis
Pear
SS-PEA SeriesTier 3
Target Trait
150K
SHU
Reported target: 150K SHU
Technical Specifications
Genomic Edits
12
GC Content
(46%)
Construct Length
8,600 bp
Promoter System
EF1α constitutive
Expression System
pLenti-CMV cationic lipid nanoparticle delivery
Host Strain
Cationic lipofection suspension culture LC-50
Pathway Edits
- De novo capsaicin pathway 7-gene cassette insertion via T2A polyprotein
- CYP450 cassette with P450 reductase CPR1 co-expression (1:1 ratio)
- ABC transporter ABCG16 knock-in for vacuolar product export
- Vacuolar sequestration module VSM1 integration
Key Interventions
T2A self-cleaving polyprotein single-cassette deliveryCPR1 co-expression at 1:1 stoichiometry with CYP450 cassette
Application Domains
Food ManufacturingAgricultural Biotech
Intended Use
Delivers Pear-platform capsaicinoids at 150K SHU for scalable food-grade heat ingredient manufacturing.
Access
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