Apple T4-CR-RS

Malus domestica

Apple

SS-APL SeriesTier 3

Target Trait

75K

SHU

Reported target: 75K SHU

Technical Specifications

Genomic Edits

GC Content

(45%)

Construct Length

8,200 bp

Promoter System

U6-26 sgRNA + 35S BE4max

Expression System

SpCas9-NG base-editor ribonucleoprotein complex

Host Strain

CRISPR-RNP electroporation zygotic embryo

Pathway Edits

De novo capsaicin pathway 7-gene cassette insertion via T2A polyprotein
CYP450 cassette with P450 reductase CPR1 co-expression (1:1 ratio)
ABC transporter ABCG16 knock-in for vacuolar product export

Key Interventions

T2A self-cleaving polyprotein single-cassette deliveryCPR1 co-expression at 1:1 stoichiometry with CYP450 cassetteABCG16 vacuolar transporter for sequestration and yield protection

Application Domains

ResearchPharmaceutical

Intended Use

Enables Apple cross-species capsaicinoid biosynthesis research and pharmaceutical precursor development at 75K SHU.

Access

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