Commercial Access Only
Specimen SS-COM-BEY-1736 // BEYOND-PEPPERS
Apple T4-PR-RS
Malus domestica
Apple
SS-APL SeriesTier 3
Target Trait
75K
SHU
Reported target: 75K SHU
Technical Specifications
Genomic Edits
11
GC Content
(45%)
Construct Length
8,200 bp
Promoter System
35S CaMV PEG-optimized
Expression System
pKanII PEG-mediated transformation
Host Strain
PEG-mediated protoplast suspension culture
Pathway Edits
- De novo capsaicin pathway 7-gene cassette insertion via T2A polyprotein
- CYP450 cassette with P450 reductase CPR1 co-expression (1:1 ratio)
- ABC transporter ABCG16 knock-in for vacuolar product export
Key Interventions
T2A self-cleaving polyprotein single-cassette deliveryCPR1 co-expression at 1:1 stoichiometry with CYP450 cassetteABCG16 vacuolar transporter for sequestration and yield protection
Application Domains
ResearchPharmaceutical
Intended Use
Enables Apple cross-species capsaicinoid biosynthesis research and pharmaceutical precursor development at 75K SHU.
Access
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