Commercial Access Only
Specimen SS-COM-BEY-1714 // BEYOND-PEPPERS
Apple T2-BL-RS
Malus domestica
Apple
SS-APL SeriesTier 3
Target Trait
5K
SHU
Reported target: 5K SHU
Technical Specifications
Genomic Edits
9
GC Content
(43%)
Construct Length
7,400 bp
Promoter System
Act1 rice promoter
Expression System
pAHC17 biolistic expression cassette
Host Strain
PDS-1000/He embryogenic callus DV8
Pathway Edits
- De novo capsaicin pathway 7-gene cassette insertion via T2A polyprotein
- CYP450 cassette with P450 reductase CPR1 co-expression (1:1 ratio)
- ABC transporter ABCG16 knock-in for vacuolar product export
- Vacuolar sequestration module VSM1 integration
Key Interventions
T2A self-cleaving polyprotein single-cassette deliveryCPR1 co-expression at 1:1 stoichiometry with CYP450 cassetteABCG16 vacuolar transporter for sequestration and yield protection
Application Domains
ResearchPharmaceutical
Intended Use
Enables Apple cross-species capsaicinoid biosynthesis research and pharmaceutical precursor development at 5K SHU.
Access
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