Commercial Access Only
Specimen SS-COM-BEY-1707 // BEYOND-PEPPERS
Apple T1-CR-FD
Malus domestica
Apple
SS-APL SeriesTier 3
Target Trait
500
SHU
Reported target: 500 SHU
Technical Specifications
Genomic Edits
8
GC Content
(42%)
Construct Length
7,000 bp
Promoter System
U6-26 sgRNA + 35S BE4max
Expression System
SpCas9-NG base-editor ribonucleoprotein complex
Host Strain
CRISPR-RNP electroporation zygotic embryo
Pathway Edits
- De novo capsaicin pathway 7-gene cassette insertion via T2A polyprotein
- CYP450 cassette with P450 reductase CPR1 co-expression (1:1 ratio)
- ABC transporter ABCG16 knock-in for vacuolar product export
Key Interventions
T2A self-cleaving polyprotein single-cassette deliveryCPR1 co-expression at 1:1 stoichiometry with CYP450 cassette
Application Domains
Food ManufacturingAgricultural Biotech
Intended Use
Delivers Apple-platform capsaicinoids at 500 SHU for scalable food-grade heat ingredient manufacturing.
Access
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