Commercial Access Only
Specimen SS-COM-BEY-1673 // BEYOND-PEPPERS
Raspberry T3-BL-FD
Rubus idaeus
Raspberry
SS-RAS SeriesTier 3
Target Trait
25K
SHU
Reported target: 25K SHU
Technical Specifications
Genomic Edits
10
GC Content
(44%)
Construct Length
7,800 bp
Promoter System
CaMV 35S biolistic
Expression System
pAHC17 biolistic expression cassette
Host Strain
PDS-1000/He embryogenic callus DV8
Pathway Edits
- De novo capsaicin pathway 7-gene cassette insertion via T2A polyprotein
- CYP450 cassette with P450 reductase CPR1 co-expression (1:1 ratio)
- ABC transporter ABCG16 knock-in for vacuolar product export
- Vacuolar sequestration module VSM1 integration
- Shikimate pathway amplification via DAHPS feedback-resistant overexpression
Key Interventions
T2A self-cleaving polyprotein single-cassette deliveryCPR1 co-expression at 1:1 stoichiometry with CYP450 cassette
Application Domains
Food ManufacturingAgricultural Biotech
Intended Use
Delivers Raspberry-platform capsaicinoids at 25K SHU for scalable food-grade heat ingredient manufacturing.
Access
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